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KMID : 0380620190510040301
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Korean Journal of Food Science and Technology
2019 Volume.51 No. 4 p.301 ~ p.308
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Validation of analytical method and antioxidant properties of Eriobotrya japonica Lindl. Leaf extract according to extraction solvent
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Kim Hyun-Hee
Heo Mi-Ra
Lee Song-Mi
Yim Soon-Ho
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Abstract
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The antioxidant properties of Eriobotrya japonica leaf extract were investigated using DPPH and ABTS radical scavenging assay. The 80% ethanol extract of leaves (IC50 values for DPPH and ABTS were 13.9 and 10.9 μg/mL, respectively) and young leaves (IC50 values for DPPH and ABTS were 20.7 and 17.3 μg/mL, respectively) showed high radical scavenging activity. Additionally, the quantitative method for estimation of ellagic acid and chlorogenic acid from E. japonica leaves was optimized by HPLC/DAD. This method showed high linearity of the calibration curve with a coefficient of correlation (R2) equal to 0.999. The LOD values for ellagic acid and chlorogenic acid were 2.35 and 0.73 μg/mL, respectively, whereas LOQ values were 7.13 and 2.22 μg/mL, respectively. Recovery of the two compounds was 99.7-108.0% with RSD values less than 5.31%. These results suggest that 80% ethanol extract of E. japonica leaves could serve as a potential source of natural antioxidant for us in various industrial applications.
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KEYWORD
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Eriobotrya japonica, antioxidant, ellagic acid, chlorogenic acid, validation
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